Dr. Amit Pande, PhD is a distinguished scientist and researcher whose work is rooted in solid scientific principles and high-complexity clinical diagnostics. He holds a PhD in Biotechnology from Shri Venkateshwara University (SVU), India, with a deep specialization in molecular and clinical research.
As an Independent Scientific Advisor for Medihealth PRO, Dr. Pande lends his scientific authority to ensure that all health information is precise, verifiable, and strictly evidence-based. He bridges the critical gap between complex laboratory science and daily life, translating decades of research experience into practical, real-world guidance that empowers readers to make confident health decisions.
The Scientific Lens: From Lab to Life
Dr. Pande translates the same rigorous scientific approach required for analyzing clinical data to explain everyday health concerns. By focusing on the biological “why” behind issues—such as hormonal shifts, sleep patterns (e.g., sleep regression), chronic inflammation, and immune challenges—he connects real-world health outcomes to their cellular and molecular roots. His mission is to bridge the gap where complex medical science is often too difficult to apply practically, ensuring every piece of information you receive is simplified, understandable, and fully backed by science.
Academic Credentials & Clinical Excellence
Dr. Pande’s advanced specialization allows him to analyze health claims at the molecular and cellular levels—the foundational science behind every article on this platform.
- Primary Qualification: PhD in Biotechnology from Shri Venkateshwara University (SVU), India.
- Healthcare Management: Post Graduate Diploma in Hospital and Health Management & Clinical Research from Symbiosis International University, Pune, India.
- ISO 15189 (2022) Trained: Specialized in the International Standard for Medical Laboratories, ensuring quality management, technical competence, and clinical risk assessment from the National Institute of Training for Standardization (NITS), Bureau of Indian Standards.
Advanced Industry-Standard Technical Training
Dr. Pande has completed intensive, hands-on training delivered by Manufacturer Application Specialists for world-leading diagnostic platforms:
1. Molecular Genetics Analyzer
- Platform Expertise: Roche Molecular Systems– Advanced clinical training in high-throughput Real-Time PCR workflows. Expertise includes automated nucleic acid extraction and the application of Roche-specific diagnostic assays for infectious diseases and oncology.
- Instrument Expertise: Cobas® 5800 system & Cobas s 201.
2. Immunogenetics Analyzer
- Platform Expertise: Luminex xMAP® Technology– Hands-on proficiency in multiplexed bead-based assays. Specialized in Human Leukocyte Antigen (HLA) typing and the detection of Donor-Specific Antibodies (DSA) to monitor and prevent transplant rejection.
- Instrument Expertise: Luminex 200 uses xMAP® Technology.
3. Genomic Sequencing (NGS & Sanger Sequencer)
- Platform Expertise: Thermo Fisher Scientific– Certified technical training in Next-Generation Sequencing workflows, including library preparation, template generation, and the interpretation of complex genomic data for personalized medicine.
- Instrument Expertise: Ion Torrent™ and Sanger Sequencer.
4. Flow cytometry
- Platform Expertise: BD (Becton Dickinson) Biosciences Certified technical training in multi-color panel design, fluidics optimization, and clinical workflows for immunophenotyping, lymphocyte crossmatch and the characterization of hematopoietic stem cells (CD34+).
- Instrument Expertise: BD FACSCanto™ II Clinical Flow Cytometry System, BD FACSVerse™ Flow Cytometer & BD FACS Calibur.
Core Expertise
Dr. Pande translates a rigorous clinical approach to explain everyday health concerns, uncovering the biological “why” behind chronic issues.
He connects clinical data, diagnostic markers, and research evidence to real-life health outcomes, helping readers understand not just what is happening in their bodies, but why it is happening. As a result, complex medical science becomes practical, actionable, and easier to apply in daily health decisions.
1. Immunology & Transplantation
Advanced assessment of systemic immune responses and transplant rejection risks. Dr. Pande utilizes Luminex xMAP and Thermo Fisher (One Lambda) technologies to analyze HLA antibodies and Donor-Specific Antibodies (DSA).
2. Hematology & Transfusion Medicine
Expertise in the clinical complexities of leucodepletion and transfusion-related alloimmunization. He evaluates how blood-based cellular changes impact systemic immunity and long-term health outcomes.
3. Genomics & Molecular Diagnostics
Mastery of high-complexity platforms, including Next-Generation Sequencing (NGS) and Real-Time PCR (qPCR) via Roche systems (5800). Dr. Pande specializes in interpreting genomic blueprints to provide precise, personalized health insights.
4. Cell Signaling & Endocrinology
Decoding the biochemical pathways and hormonal signaling mechanisms that facilitate cellular communication. This expertise is critical for understanding metabolic balance, stress response, and endocrine health.
5. Microbiology & The Microbiome
Scientific analysis of microbial communities and their profound influence on gut health, chronic inflammation, and immune modulation.
6. Regenerative Medicine
Deep understanding of stem cell biology and translational applications. He specializes in the characterization of hematopoietic stem cells (CD34+) and the monitoring of immune reconstitution through T-cell subset analysis (CD3, CD4, and CD8).
7. Clinical Research & Data
Applying ISO 15189 standards to evidence-based research. Dr. Pande specializes in the rigorous interpretation of complex molecular data to filter medical facts from health myths.
Verifiable Authority: Peer-Reviewed Publications
Dr. Pande has authored or co-authored over 50 peer-reviewed international research papers in specialized fields such as Renal Transplantation and Transfusion Medicine. This extensive track record establishes the clinical integrity and rigor he brings to the editorial process.
Selected Publications & Research Portfolio
- A retrospective observational study to estimate the risk of HLA alloimmunization with blood transfusion: Can the risk be reduced by leucodepletion? Immunobiology. 2023. View on ScienceDirect ↗︎
- Detection of donor-specific HLA antibodies: A retrospective observation in 350 renal transplant cases. Transpl Immunol, 2023. View on PubMed ↗︎
- Effects of Different Sensitization Events on HLA Alloimmunization in Renal Transplant Cases; a Retrospective Observation in 1066 Cases. Transplant Immunology, 2022. View on PubMed ↗︎
- Significance of Luminex-crossmatch assay and its mean fluorescence intensity-a retrospective observation in 380 renal transplant cases. Polish journal of surgery, 2022. View on Polish Journal of Surgery. View on Polish Journal of Surgery ↗︎
- A Case of Successful Desensitization in High Risk HLA Incompatible Kidney Transplantation. Indian J Nephrol, 2021. View on Science Direct ↗︎
- Clinical outcome of living donor kidney transplantation across simultaneous ABO and HLA incompatibility: Single center experience of first ten cases. Transfus Apher Sci, 2021. View on PubMed ↗︎
- Desensitization Therapy in Kidney Transplantation Cases with Positive Baseline Complement-Dependent Cytotoxicity Crossmatch and High Donor-Specific Antibodies: A Retrospective Study. Indian Journal of Transplantation, 2021. Indian Journal of Transplantation ↗︎
- Outcome of desensitization in human leukocyte antigen and ABO incompatible living donor kidney transplantation: Single center experience of first 200 incompatible transplants. J Clin Apher, 2021. Journal of Clinical Apheresis↗︎
- Renal transplantation in HLA sensitized patients: Traversing the immunological barrier. Therapeutic Apheresis and Dialysis, 2020. View on PubMed ↗︎
- Comparative Study for Measurement of Residual Leucocytes in Leuco-depleted Red Blood Cells by Two Different Methods. Indian journal of hematology and blood transfusion, 2020. View on PubMed ↗︎
- Extraoral plasmablastic lymphoma detected using ascitic fluid cytology and flow cytometry: a case report with a review of the literature. Acta Cytol. 2014;58(3):309-17. View on PubMed ↗︎
- Stability of eosin-5′-maleimide dye used in flow cytometric analysis for red cell membrane disorders. Blood Res. 2015 Jun;50(2):109-12. View on PubMed ↗︎
- CD5 positive hairy cell leukemia: A rare case report with brief review of literature. Cytometry B Clin Cytom. 2016 Sep;90(5):467-72. View on PubMed ↗︎
- Expression of CD71 by flow cytometry in acute leukemias: More often seen in acute myeloid leukemia. Indian J Pathol Microbiol. 2016 Jul-Sep;59(3):310-3. View on PubMed ↗︎
- Comparative analysis of complement-dependent lymphocytotoxicity crossmatch and flow cytometry crossmatch results versus Luminex single-antigen bead-based donor-specific IgG class I antibody MFI values in live related renal transplant cases; a retrospective observation in 102 cases. J Immunoassay Immunochem. 2021 May 4;42(3):300-313. View on PubMed ↗︎
- Hepatosplenic T Cell Lymphoma in an Immunocompetent Female Diagnosed using Flow Cytometry: A Rare Clinical Entity. J Clin Diagn Res. 2016 Aug;10(8):ED15-6. View on PubMed ↗︎
- Aberrant NK cell associated marker (CD56 and CD57) expression in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2015 Sep-Oct;88(5):348-51. View on PubMed ↗︎
- Detection of T and B cells specific complement-fixing alloantibodies using flow cytometry: A diagnostic approach for a resource limited laboratory. Asian J Transfus Sci. 2017 Jul-Dec;11(2):171-179. View on PubMed ↗︎
- Primary follicular lymphoma of the breast: a rare clinical entity diagnosed using tissue flow cytometry. Indian J Hematol Blood Transfus. 2015 Jun;31(2):300-1. View on PubMed ↗︎
- Renal transplantation in HLA sensitized patients: Traversing the immunological barrier. Ther Apher Dial. 2020 Oct;24(5):578-590. View on PubMed ↗︎
Verifiable Research Profiles
To view Dr. Pande’s complete research history, please visit his official profiles: View My Research Profile on :
⚠️Medical Disclaimer: Dr. Amit Pande’s content on medihealth PRO is for informational and educational purposes only and is not a substitute for professional medical diagnosis, treatment, or advice. Always consult with a qualified healthcare provider.